Vine maturity
‘Premier Russet’ has a late vine maturity and ‘Rio Grande Russet’ has a medium late maturity. 0–6.5) than ‘Premier Russet’ (6.0–7.5). For the progeny, mean trait values displayed a wide range of phenotypic variation (Additional file 4: Table S3), with a high correlation between years (> 0.70, P < 0.001) and across environments (> 0.55, P < 0.001). The broad-sense heritability for both years at each location was statistically significant, with H 2 estimates ranging from 0.83 to 0.93 (Table 2). These results were consistent for all vine maturity traits. When mean values for maturity were mapped to the linkage maps, a significant association was detected on chromosome 5 (Fig. 2, Additional file 6: Figure S2). This association was consistent between years and environments. Logarithm of the odds (LOD) scores ranged from of 21.2 and 34.1, well above the permutation thresholds. Collectively, they explained between 38 and 54% of the trait variance (Table 3). The peak of the LOD profile was consistently detected at 15.0 cM (Table 3, Fig. 2). The best simple model for maturity was a double-simplex configuration (AAAB ? AAAB) on homologous chromosomes H2 and H8 of Rio Grande russet (RGR) and Premier Russet (PR), respectively (Additional file 6: Figure S2). This model implies a QTL with allele effects from both parents, and with the B allele associated with earlier maturity (P < 0.001). Thus, progeny with two copies of the B allele (AABB) had on average earlier maturity (3.1–4.0) than progeny with a single copy (AAAB, 4.5–6.2) or lacking the B allele (AAAA, 6.4–7.5).
LOD get distribution away from QTLs having attributes during the maturity locus part into the chromosome 5. For every single panel on shape means a different sort of trait. LOD threshold 95% count on times is placed in Table step 3. California, citric acidic 2010; EB, very early blight; FBE, bud-stop fry color Idaho; GH, growth habit; GLU, glucose; M95, vine maturity at the 95DAP; M120, vine maturity within 120DAP; TSHA, tuber profile; VW, Verticillium wilt; ID, Idaho; NC, North carolina; DAP, weeks immediately after planting; numbers ten and 11 regarding feature brands suggest decades 2010 and you may 2011, correspondingly
New SNP closest toward QTL for maturity which had an excellent double-simplex arrangement are solcap_snp_c2_11605, located at 9.step one cM. Regression of one’s maturity imply philosophy into the genotype of this SNP told me between 38 and you will 50% of the phenotypic variance. The level of the LOD bend (15 cM) are organized contained in this a gap between indicators you to definitely spanned 8 to 10 cM, to your solcap_snp_c2_11605 situated on one side of the gap. This place ports this new Solanum tuberosum cycling DOF grounds gene step 1 (StCDF1, PGSC0003DMG400018408), that has been said to be a primary regulator away from potato bush maturity . Visual image of one’s genetic and you will physical (Mb) range out-of homologous chromosomes H2 and H8 presented the fresh new requested bend figure and you will fit better having chromosome construction indicating a high training from concordance between your genetic condition (cM) and bodily place (Mb) (Extra file 7: Figure S3).
Several qualities co-localize within maturity locus part on chromosome 5
Eight faculties and citric acid (CANC10), progress routine (GHID, GHNC), bud-stop fry colour (FBEID), very early blight get (EBID11), tuber sugar (GLUID11), tuber figure (TSHAID), and you can Verticillium wilt score (VWID11) co-nearby with vine readiness traits (MAT95ID, MAT95NC, MAT120ID, MAT120NC) to the chromosome 5 (Table step 3, Fig. 2). The best genetic design for everybody qualities, but GLUID11 and FBEID was a double-simplex effect on H2 and you will H8 to the SNP, solcap_snp_c2_11605, that have so it setting (Table step three). If residuals regarding regression of all the six faculties into vine readiness was indeed analyzed, no high effectation of a beneficial QTL are located for EBID11, TSHAID, and you may GHNC. To possess gains routine (GHID) and you can VWID11, a serious QTL are noticed only if fixed for maturity during the 95DAP however, was not extreme at 120DAP. Research of glucose concentrations (GLUID11) presented a LOD off 3.nine from the 21 cM, with good QTL impact on H2 and you can H5. eros escort Concord Immediately following modifying having maturity, a peak on the LOD bend to own GLUID11 try observed only below the 95% permutation threshold.